流式细胞术
癌症研究
下调和上调
细胞凋亡
竞争性内源性RNA
生物
脂质代谢
细胞生长
长非编码RNA
细胞生物学
分子生物学
生物化学
基因
作者
Qing Zhou,Deshui Kong,Wenzhi Li,Zhengzheng Shi,Yao Liu,Rui Sun,Xiaohong Ma,Chunping Qiu,Zhiming Liu,Yixin Hou,Jie Jiang
出处
期刊:Life Sciences
[Elsevier]
日期:2023-05-01
卷期号:320: 121512-121512
被引量:2
标识
DOI:10.1016/j.lfs.2023.121512
摘要
Endometrial cancer (EC) is a malignant tumor with a high incidence in women, and the survival rate of high-risk patients decreases significantly after disease progression. The regulatory role of long non-coding RNAs (LncRNAs) in tumors has been widely appreciated, but there have been few studies in EC. To investigate the effect of HOXB-AS3 in EC, we used bioinformatics tools for prediction and collected clinical samples to detect the expression of HOXB-AS3. Colony formation assay, MTT assay, flow cytometry and apoptosis assay, and transwell assay were used to verify the role of HOXB-AS3 in EC. HOXB-AS3 was upregulated in EC, promoted the proliferation and invasive ability of EC cells, and inhibited apoptosis. In addition, the ROC curve illustrated its diagnostic value. We explored experiments via lentiviral transduction, FISH, Oil Red O staining, TC and FFA content detection, RNA-pulldown, RIP, and other mechanisms to reveal that HOXB-AS3 can bind to PTBP1 and co-regulate the expression of SREBP1, thereby regulating lipid metabolism in EC cells. To the best of our knowledge, this is the first study on HOXB-AS3 in disorders of lipid metabolism in EC. In addition, we believe HOXB-AS3 has the potential to be a neoplastic marker or a therapeutic target.
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