Guidelines for Applying Gene Mutagenesis Methods in Organic Chemistry, Pharmaceutical Applications, and Biotechnology

In Chapter 3, different mutagenesis approaches in directed evolution and rational design are introduced in greater detail than in Chapter 1. Readers may wonder which technique should be applied to a particular protein engineering task. Herein, this chapter gives some useful and general tips when applying rational design and directed evolution. In parallel, a description of comparative studies and recent developments is provided as well. The future trend in protein engineering is the fusion of rational design and directed evolution. An excellent example is the emergence of Focused Rational Iterative Site-specific Mutagenesis (FRISM).