Impact of the quality of resected thyroid cancer tissue sample on next‐generation sequencing testing

Abstract Activating rearranged during transfection (RET) proto‐oncogene alterations can be identified using next‐generation sequencing (NGS) of tumor DNA/RNA. We assessed factors associated with NGS (Oncomine Dx Target Test [ODxTT]) success for resected thyroid cancer (TC) specimens, including sample age, processing conditions, and DNA/RNA quality. TC samples were from three Japanese hospitals, with sample age <1–<10 years, fixative 10%/15% neutralized buffered formalin (NBF), and fixation time ≤48 h/>48 h–≤72 h. NGS success rate was defined as the percentage of samples returning validated NGS results (RET fusion‐positive/negative [RNA] or RET mutation‐positive/negative [DNA], detected using ODxTT). DNA/RNA quality was assessed with indexes based on electrophoresis (DNA/RNA integrity number, DV 200 ) and quantitative polymerase chain reaction (DNA/RNA integrity score [ddCq/ΔCq]). NGS success rate ( N = 202) was 90%/93% (DNA/RNA) overall, 98%–100% (DNA and RNA) for samples <3 years old, and 91% (DNA and RNA) for samples ≥3–<5 years old fixed in 10% NBF for ≤48 h. Multivariate logistic regression analysis identified ddCq and ΔCq as significant predictors of DNA and RNA NGS success rates, respectively. Quality assessment of nucleic acid extracted from archival tissue samples is important for achieving high NGS success rates in clinical practice, especially for samples ≥3 years old.