Multistage optimal control for microbial fed-batch fermentation process

<p style='text-indent:20px;'>In this paper, we consider multistage optimal control of bioconversion glycerol to 1, 3-propanediol(1, 3-PD) in fed-batch fermentation process. To maximize the productivity of 1, 3-PD, the whole fermentation process is divided into three stages according to the characteristics of microbial growth. Stages 2 and 3 are discussed mainly. The main aim of stage 2 is to restrict accumulation of 3-hydroxypropionaldehyde and maximize the biomass in the shortest time, and the purpose of stage 3 is to get high productivity of 1, 3-PD. With these different objectives, multi-objective optimal control problems are proposed in stages 2 and 3. In order to solve the above optimal control problems, the multi-objective problems are transformed to the corresponding single-objective problems using the mass balance equation of biomass and normalization of the objective. Furthermore, the single-objective optimal control problems are transformed to two-level optimization problems by the control parametrization technique. Finally, numerical solution methods combined an improved Particle Swarm Optimization with penalty function method are developed to solve the resulting optimization problems. Numerical results show that the productivity of 1, 3-PD is higher than the reported results.</p>