Nanoarchitectonics of Fullerene‐Based Enzyme Mimics for Osteogenic Induction of Stem Cells

Enzyme mimicry is a topic of considerable interest in the development of multifunctional biomimetic materials. Mimicking enzyme activity is a major challenge in biomaterials research, and artificial analogs that simultaneously recapitulate the catalytic and metabolic activity of native enzymes are considered to be the ultimate goal of this field. This consensus may be challenged by self-assembling multifunctional nanostructures to develop close-to-fidelity enzyme mimics. Here, the ability of fullerene nanostructures decorated with active units to form enzyme-like materials that can mimic phosphatases in a metal-free manner is presented. These nanostructures self-assemble into nanoclusters forming multiple random active sites that can cleave both phosphomonoesters and phosphodiesters while being more specific for the phosphomonoesters. Moreover, they are reusable and show an increase in catalytic activity over multiple cycles similar to their natural counterparts. In addition to having enzyme-like catalytic properties, these nanocatalysts imitate the biological functions of their natural analogs by inducing biomineralization and osteoinduction in preosteoblast and mesenchymal stem cells in vitro studies.