生物神经网络
突触
突触后电位
纳米技术
轴突
材料科学
聚二甲基硅氧烷
树突棘
电子线路
轴突引导
神经科学
计算机科学
生物
物理
海马结构
受体
量子力学
生物化学
作者
José C. Mateus,Sean Weaver,Dirk van Swaay,Aline F. Renz,Julian Hengsteler,Paulo Aguiar,János Vörös
出处
期刊:ACS Nano
[American Chemical Society]
日期:2022-04-11
卷期号:16 (4): 5731-5742
被引量:6
标识
DOI:10.1021/acsnano.1c10750
摘要
Methods for patterning neurons in vitro have gradually improved and are used to investigate questions that are difficult to address in or ex vivo. Though these techniques guide axons between groups of neurons, multiscale control of neuronal connectivity, from circuits to synapses, is yet to be achieved in vitro. As studying neuronal circuits with synaptic resolution in vivo poses significant challenges, we present an in vitro alternative to validate biophysical and computational models. In this work we use a combination of electron beam lithography and photolithography to create polydimethylsiloxane (PDMS) structures with features ranging from 150 nm to a few millimeters. Leveraging the difference between average axon and dendritic spine diameters, we restrict axon growth while allowing spines to pass through nanochannels to guide synapse formation between small groups of neurons (i.e., nodes). We show this technique can be used to generate large numbers of isolated feed-forward circuits where connections between nodes are restricted to regions connected by nanochannels. Using a genetically encoded calcium indicator in combination with fluorescently tagged postsynaptic protein, PSD-95, we demonstrate functional synapses can form in this region.
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