洗脱
色谱法
聚丙烯酰胺凝胶电泳
化学
电泳
分辨率(逻辑)
凝胶电泳
聚丙烯酰胺
蛋白质凝胶电泳
生物化学
酶
计算机科学
高分子化学
人工智能
出处
期刊:Methods in Enzymology
日期:2009-01-01
卷期号:: 565-572
被引量:23
标识
DOI:10.1016/s0076-6879(09)63032-9
摘要
New protein biochemical technologies have been developed that allow one to learn more and more about a protein with less and less material (on the order of microg). Polyacrylamide gel electrophoresis, which was originally strictly an analytical method, has in some cases become a high-resolution preparative method. This chapter will focus on the elution of proteins from SDS gels, with an emphasis on recovering enzyme or biological activity of the resulting protein.
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