作者
Michael Costanzo,Benjamin VanderSluis,Elizabeth N. Koch,Anastasia Baryshnikova,Carles Pons,Guihong Tan,Wen Wang,Matej Ušaj,Julia Hanchard,Susan D. Lee,Vicent Pelechano,Erin B. Styles,Maximilian Billmann,Jolanda van Leeuwen,Nydia Van Dyk,Zhen Yuan Lin,Elena Kuzmin,J. Daniel Nelson,Jeff S. Piotrowski,Tharan Srikumar,Sondra Bahr,Yiqun Chen,Raamesh Deshpande,Christoph F. Kurat,Sheena C. Li,Li Z,Mojca Mattiazzi Ušaj,Hiroki Okada,Natasha Pascoe,Bryan Joseph San Luis,Sara Sharifpoor,Emira Shuteriqi,Scott W. Simpkins,Jamie Snider,Harsha Garadi Suresh,Yizhao Tan,Hongwei Zhu,Noël Malod-Dognin,Vuk Janjić,Nataša Pržulj,Olga G. Troyanskaya,Igor Štagljar,Tian Xia,Yoshikazu Ohya,Anne‐Claude Gingras,Brian Raught,Michael Boutros,Lars M. Steinmetz,Claire Moore,Adam P. Rosebrock,Amy A. Caudy,Chad L. Myers,Brenda Andrews,Charles Boone
摘要
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to protein complexes and pathways, biological processes, and cellular compartments. Negative interactions connected functionally related genes, mapped core bioprocesses, and identified pleiotropic genes, whereas positive interactions often mapped general regulatory connections among gene pairs, rather than shared functionality. The global network illustrates how coherent sets of genetic interactions connect protein complex and pathway modules to map a functional wiring diagram of the cell.