A Tryptophanyl‐tRNA Synthetase/tRNA Pair for Unnatural Amino Acid Mutagenesis in E. coli

Selected: A tryptophanyl-tRNA synthetase(TrpRS)/tRNA pair was evolved to genetically encode tryptophan analogues and other unnatural amino acids with large side chains in E. coli. A selection scheme was employed to identify TrpRS variants able to selectively charge tRNACUATrp with Trp analogues (see picture). Substitution of Trp66 in enhanced cyan fluorescent protein (ECFP) with these Trp analogues afforded ECFP variants with novel spectral properties. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.