Examination on Activity and Stability of the Immobilizated Lipase by Chitosan Res

The method: lipase was immobilized with 0.25% glutaraldehyde and chitosan as across-linking carrier,and the catalytic activity and stability of immobilized lipase were studied.The results:the optimal conditions of highest activity of immobilized lipase were that pH was between 6.5～7.0,and temperature was between 30～50℃.Both the immobilized and dissociated lipase showed the highest activity at 40℃,but the activity of immobilized lipase was not significantly different(P0.05) between 30～50℃,and the dissociated lipase was significantly different(P0.05) at the same temperature.Immobilized lipase gradually released lipase in 0.9% NaCl solution.After immobilized lipase used at first 5 times, activity of the immobilized lipase changed little(p0.05).Conclusions: highest activity of immobilized lipase with 0.25% glutaraldehyde and chitosan as across-linking carrier had much larger range of the optimal temperature and pH than that of the dissociated lipase.