To observe the anesthetic effect of ketamine in combination with sumianxin Ⅱ in laboratory minipigs,12 laboratory minipigs(11.33 kg±0.83 kg)were anesthetized by using ketamine and sumianxin Ⅱ(0.28 mL/kg,im), and analgesic effect,breathing frequency (BR),heart rate (HR),and adverse reaction such as dribbling and disgorging were observed. 100% of the minipigs were anesthetized in (4.2±1.6) min, and anesthetic condition maintained for (87.3±15.4) min. The BR and HR of pigs during operation were stable.The results showed that it was a good anesthesia method using ketamine in combination with sumianxin Ⅱin laboratory minipigs,and the anesthesia process and vital signs were stable. So it is a conveniental and safe method to anesthetize minipigs using ketamine in combination with sumianxin Ⅱ.