Collagen Distribution and its Quantitative Variation in the Aortic Adventitia of Spontaneously Hypertensive Rats

Objective: To study collagen distribution and its quantitative variation in the aortic adventitia of spontaneously hypertensive rats Methods: Sirus red stain was used to observe collagen distribution in aortic adventitia;Chloramine\|T oxidation assay was employed to quantitate the collagen of aortic adventitia;Immuno\|histochemical staining was adopted to detect type Ⅰ、Ⅲ collagen synthetic characteristic of vascular adventitial fibroblasts. Results: Collagen of rat aoritc wall mainly distributes in adventitia;SHR shows more collagen deposition in adventitia than WKY at the age of 8weeks and 24 weeks;The immuno\|histochemical staining of type Ⅰ、Ⅲ collagen in vascular adventitial fibroblasts is positive. Conclusions: All those results indicate that vascular adventitia may be involved in hypertensive vascular remodeling.and vascular adventitial fibroblasts can synthesize collagen type Ⅰ and Ⅲ.