Microfluidic System for Rapid Detection of Airborne Pathogenic Fungal Spores

Airborne fungi, including Aspergillus species, are the major causes of human asthma. Direct capture and analysis of pathogenic fungi in indoor air is important for disease prevention and control. In this paper, we demonstrated an integrated microfluidic system capable of enrichment and high-throughput detection for airborne fungal spores of Aspergillus niger, a well-known allergenic harmful species. The microfluidic system allowed semiquantitative detection of Aspergillus niger spores based on immunofluorescence analysis. To assess its contaminated level, the whole analysis time could be completed in 2-3 h including ∼1 h of enrichment and ∼1 h of target detection. The detection limit was ∼20 spores, equivalent to ∼300 spores·m^-3 of the concerned targets in air. In addition, the microfluidic system has integrated sampling and sample analysis to avoid additional sample concentration step, showing the potential for point-of-care detection for other pathogenic fungal spores.