Co-metabolic degradation of tetrabromobisphenol A by Pseudomonas aeruginosa and its auto-poisoning effect caused during degradation process

In this study, Pseudomonas aeruginosa was applied to degrade tetrabromobisphenol A (TBBPA) with glucose as a co-metabolic substrate. Influencing factors of co-metabolic degradation such as pH, TBBPA and glucose concentration were examined and the degradation efficiency under optimal condition reached about 50% on the 7th day. The study also proved that the extracellular action, rather than intracellular one, played a leading role in TBBPA degradation. Five metabolites including debromination and beta-scission products were identified in this study. The extracellular active substance pyocyanin was considered as the origin of H2O2 and OH·. The variation of concentrations of H2O2 and OH· shared the same trend, they increased in the early days and then declined gradually. On the 1st day, the OD600 of P.aeruginosa in the co-metabolic group was 6.0 times higher than the initial value while total organic carbon (TOC) decreased about 78%, which might lead to the occurrence of pyocyanin auto-poisoning. Flow cytometry was applied to detect the cellular state of P.aeruginosa during degradation. The increasing intracellular ROS showed that cells were suffering from oxidative stress and the change of membrane potential revealed that cellular dysfunction had occurred since the 1st day. This research indicated that the toxic effect on P.aeruginosa was probably not directly correlated with TBBPA, but was caused by pyocyanin auto-poisoning.