间质细胞
生物
肿瘤微环境
结直肠癌
体细胞
癌症研究
癌相关成纤维细胞
免疫系统
癌症
免疫学
遗传学
基因
作者
Yuan Zhou,Shuhui Bian,Xin Zhou,Yueli Cui,Wendong Wang,Lu Wen,Limei Guo,Wei Fu,Fuchou Tang
出处
期刊:Cancer Cell
[Elsevier]
日期:2020-12-01
卷期号:38 (6): 818-828.e5
被引量:148
标识
DOI:10.1016/j.ccell.2020.09.015
摘要
To what extent stromal cells in the tumor microenvironment (TME) are transformed by colorectal cancer (CRC) cells is unexplored. To dissect alterations in these non-malignant cells, we performed single-cell multiomics sequencing of 21 patients with microsatellite-stable CRCs and 6 cancer-free, elderly individuals. Surprisingly, somatic copy number alterations (SCNAs) are prevalent in immune cells, fibroblasts, and endothelial cells in both the TME and the normal tissues of each individual. Moreover, the proportions of fibroblasts with SCNAs in tumors (11.1%–47.7%) are much higher than those in adjacent normal tissues (1.1%–10.6%), with gain of chromosome 7 strongly enriched in the TME, clearly indicating clonal expansion. Furthermore, five genes (BGN, RCN3, TAGLN, MYL9, and TPM2) are identified as fibroblast-specific biomarkers of poorer prognosis of CRC. Our study provides evidence and functional relevance of pervasive genomic alterations in the stromal cells of TME in CRC.
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