Effects of bactocell probiotic (Pediococcus acidilactici) and folic acid on immune system on ship sturgeon fingerling

T feeding trial was carried out to evaluate the effects of different dietary commercial probiotic (pediococcus accidilactici) and folic acid on the immune system in Ship sturgeon (Acipenser nudiventiris). 210 fish averaging 12.84±1.53 (mean SD) were randomly distributed into each of twenty one rectangular plastic tanks of 50 L capacity. Each tank was then randomly assigned to one of three replicates of seven diets containing control diet, 1(4 mg folic acid kg-1 diet), 2(300 g probiotics Tone-1 diet), 3(4 mg folic acid kg-1 diet+200 g Tone-1 diet), 4 (2 mg folic acid kg-1 diet+300 g Tone-1 diet), 5(2 mg folic acid kg-1 diet+200 g Tone-1 diet), 6 (4 mg folic acid kg-1 diet+300 g Tone-1 diet). At the end of eighteen weeks of feeding trial, both haematological indices and leucocyte counts were significantly (P < 0.05) affected by dietary pediococcus accidilactici and Folic acid and their combinations on diet. In the group with 4 mg FA+3 g pediococcus accidilactici, lysozyme activity and total immunoglobulin (Ig) level significantly increased compared to the those groups fed other diets (P < 0.05). Moreover, the fish fed with diets supplemented with 2 mg FA+ 3 g pediococcus accidilactici per kg-1 diet had considerably higher immunoglobulin (IgM) level (P < 0.05). Under the experimental conditions, dietary pediococcus accidilactici and FA had a synergistic effect on enhancing growth performance and immunity of Acipenser nudiventris.