雷公藤甲素
MCF-7型
细胞周期
细胞凋亡
雷公藤
癌细胞
化学
雌激素受体
癌症研究
细胞生长
细胞培养
细胞周期检查点
细胞
分子生物学
乳腺癌
癌症
内科学
生物
医学
生物化学
人体乳房
病理
替代医学
遗传学
作者
Jing Liu,Zhenzhou Jiang,Jingwei Xiao,Yun Zhang,Sensen Lin,Weigang Duan,Jincheng Yao,Chunhui Liu,Xin Huang,Tao Wang,Zhongliang Liang,Rongrong Wang,Shuang Zhang,Luyong Zhang
出处
期刊:Phytomedicine
[Elsevier]
日期:2009-11-01
卷期号:16 (11): 1006-1013
被引量:45
标识
DOI:10.1016/j.phymed.2009.03.021
摘要
The aim of the study was to discover possible differential cytotoxicity of triptolide towards estrogen-sensitive MCF-7 versus estrogen-insensitive MDA-MB-231 human breast cancer cells. Considering that MCF-7 cells express functional Estrogen receptor α (ERα) and wild-type p53, whereas MDA-MB-231 cells which are ERα-negative express mutant p53, the anti-proliferation effect of triptolide on MCF-7 and MDA-MB-231 cells were examined, the apoptotic effect and cell cycle arrest caused by triptolide were investigated, ERα and p53 expression were also observed in this paper. The results showed that the anti-proliferation effects were induced by triptolide in both cell lines. But the value of IC50 in MCF-7 cells for its anti-proliferation effect was about one tenth of that in MDA-MB-231 cells, which indicated that the effect is more potent in MCF-7 cells. Condensed chromatin or fragmented nuclei could be found in MCF-7 cells treated with only 40 nM triptolide but in MDA-MB-231 cells they couldn’t be observed until the concentration reached to 400 nM. Triptolide induced significant S cell cycle arrest along with the presence of sub-G0/G1 peak in MDA-MB-231 cells, whereas there was only slightly S cell cycle arrest on cell cycle distribution in MCF-7 cells. The role of p53 in two breast cancer cells was examined, the results showed that the mutant p53 in MDA-MB-231 cells was suppressed and the wild-type p53 in MCF-7 was increased. Moreover, triptolide could down regulate the expression of ERα in MCF-7 cells. The results showed that triptolide is much more sensitive to ERα-positive MCF-7 cells than to ERα-negative MDA-MB-231 cells, and the sensitivity is significantly associated with the ERα and p53 status.
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