荧光相关光谱
反褶积
荧光
光谱学
化学
荧光光谱法
扩散
化学物理
激发
荧光互相关光谱
稳健性(进化)
生物系统
分析化学(期刊)
生物物理学
波长
作者
Sonali A. Gandhi,Matthew A. Sanders,James G. Granneman,Christopher V. Kelly
标识
DOI:10.1016/j.bpj.2021.11.2360
摘要
The dynamics of lipids and proteins are crucial in the mechanisms of cellular functions. The robustness of fluorescence correlation spectroscopy (FCS) makes it possible to quantify the diffusion and molecular interactions at nanomolar concentration in biological systems. We developed novel, economical methods with the use of super-continuum laser and spectral deconvolution with experiment-specific excitation wavelengths and emission spectra for revealing the interactions between up to four spectrally overlapping fluorophores.
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