Chitin/corn stalk pith sponge stimulated hemostasis with erythrocyte absorption, platelet activation, and Ca2+-binding capabilities

Chitin (CT) is widely used as a hemostatic material in surgical sponges, although its efficacy needs improvement to promote the clotting process. In this study, another green biomass, corn stalk pith (CSP), was incorporated into CT through ball milling to fabricate CT-CSP composite hemostatic sponges to enhance erythrocyte absorption, platelet activation, and clotting factor accumulation (Ca2+). In vitro hemostatic analysis indicated that CSP incorporation greatly promoted the coagulation process, with a much lower blood clot index and higher blood clot stability. In addition, the composite sponge promoted more platelet adhesion and activation, and the composite sponge demonstrated a greater ability to bind clotting factors (Ca2+). Consistently, it achieved complete hemostasis with less blood loss and a shorter hemostatic time in a rat liver injury-model. This composite hemostatic sponge is sustainable, cost-efficient, and biocompatible, which highlight the excellent translational potential in clinical settings.