多糖
香菇属
单糖
化学
半乳糖
甘露糖
凝胶渗透色谱法
细胞毒性
分子质量
生物化学
高效液相色谱法
色谱法
体外
食品科学
有机化学
蘑菇
酶
聚合物
作者
Yan Zheng,Miao Huang,Hongmei Nan,Iteku Bekomo Jeff,Yifa Zhou,Yufei Gao
出处
期刊:PubMed
日期:2015-05-01
卷期号:28 (3 Suppl): 1069-74
被引量:3
摘要
Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC). The fractions were analyzed for monosaccharide composition, molecular weight, and then tested for cytotoxicity activity. The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios. All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml. The antitumor activities of the polysaccharides were related to their monosaccharide content and molecular weight. The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible. The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
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