ABSTRACT Objective Examine the relationship between parenting stress and thriving at work among nurses. Examine whether organizational support mediates the association between parenting stress and thriving at work among nurses. Methods A questionnaire survey was conducted with 534 clinical nurses using the Parenting Stress Scale for Clinical Nurses, the Thriving at Work Scale, and the Perceived Organizational Support Questionnaire. Results The mean total scores for nursing parenting stress, perceived organizational support, and the Thriving at Work Scale were 2.72 ± 0.47, 3.63 ± 0.81, and 4.80 ± 0.74, respectively. A significant negative correlation was observed between nursing parenting stress and thriving at work (r1 = −0.233, p < 0.001) and between nursing parenting stress and perceived organizational support (r2 = −0.289, p < 0.001). A significant positive correlation was also observed between perceived organizational support and thriving at work (r3 = 0.469, p < 0.001). Perceived organizational support partially mediated the relationship between parenting stress and thriving at work, accounting for 52.72% of the total effect. Conclusion This study supports the mediating role of perceived organizational support in the relationship between parenting stress and thriving at work among nurses. Interventions to promote perceived organizational support among nurses with parenting stress should be studied to improve their well-being.