粟粒圆锥花序
淀粉
支链淀粉
生物
转录组
直链淀粉
糖
淀粉合成酶
植物
玉米淀粉
食品科学
基因
生物化学
基因表达
作者
Qinghua Yang,Yuhao Yuan,Jiajia Li,Mengru Han,Jing Li,Fei Jin,Baili Feng
标识
DOI:10.1016/j.ijbiomac.2023.123155
摘要
Broomcorn millet is a popular cereal with health benefits, and its grains are rich in starch. However, the differences in the pathway and key genes involved in starch biosynthesis of waxy and non-waxy broomcorn millet grain remain unclear. Therefore, the grain and starch physicochemical index and transcriptomic analyses of two genotypes of broomcorn millet were conducted at 3, 6, 9, 12, 15, 18, and 21 days after pollination. The phenotypic and physiological results indicated that the starch synthetic process of non-waxy and waxy broomcorn millet was significantly different. The amylose, amylopectin, and total starch contents of non-waxy broomcorn millet were 1.99, 4.74, and 6.73 mg/grain, while those of waxy broomcorn millet were 0.34, 5.94, and 6.28 mg/grain, respectively. The transcriptomic analysis revealed that 106 differentially expressed genes were identified, which were mainly enriched in the “amino sugar and nucleotide sugar metabolism”, “pyruvate metabolism”, “galactose metabolism”, and “starch and sucrose metabolism” pathways. The WGCNA suggested that a total of 31 hub genes were correlated with starch biosynthesis. These findings provide a new approach to studying the starch synthesis in broomcorn millet.
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