明胶
纳米纤维
伤口愈合
静电纺丝
聚乙烯醇
材料科学
生物相容性
乙醇酸
生物医学工程
化学
乳酸
外科
纳米技术
医学
复合材料
聚合物
生物化学
冶金
生物
细菌
遗传学
作者
Giriraj Pandey,Prashant Pandey,Dilip Kumar Arya,Shubham Kanaujiya,Deshraj Deepak Kapoor,Ravi Gupta,Shivendu Ranjan,Kumarappan Chidambaram,Balamurugan Muthukutty,Paruvathanahalli Siddalingam Rajinikanth
标识
DOI:10.1016/j.ijpharm.2023.122918
摘要
Electrospun nanofibers scaffolds show promising potential in wound healing applications. This work aims to fabricate nanofibrous wound dressing as a novel approach for a topical drug delivery system. Herein, the electrospinning technique is used to design and fabricate bioabsorbable nanofibrous scaffolds of Polyvinyl alcohol/gelatin/poly (lactic-co-glycolic acid) enriched with thrombin (TMB) as hemostatic agent and vancomycin (VCM) as anti-bacterial agent for a multifunctional platform to control excessive blood loss, inhibit bacterial growth and enhance wound healing. SEM, FTIR, XRD, in vitro drug release, antimicrobial studies, biofilm, cell viability assay, and in vivo study in a rat model were used to assess nanofiber's structural, mechanical, and biological aspects. SEM images confirms the diameter of nanofibers which falls within the range from 150 to 300 nm for all the batches. Excellent swelling index data makes it suitable to absorb wound exudates. In-vitro drug release data shows sustained release behavior of nanofiber. Nanofibers scaffolds showed biomimetic behavior and excellent biocompatibility. Moreover, scaffolds exhibited excellent antimicrobial and biofilm activity against Staphylococcus aureus. Nanofibrous scaffolds showed less bleeding time, rapid blood coagulation, and excellent wound closure in a rat model. ELISA study demonstrated the decreasing level of inflammatory markers, such as TNF-α, IL1β, and IL-6, making formulation promising for hemostatic wound healing applications. Finally, the study concludes that nanofibrous scaffolds loaded with TMB and VCM have promising potential as a dressing material for hemostatic wound healing applications.
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