区域选择性
生物催化
氰化物
基质(水族馆)
化学
组合化学
合理设计
催化作用
有机化学
生化工程
材料科学
纳米技术
反应机理
工程类
海洋学
地质学
作者
Michael Hinzmann,Hilmi Yavuzer,Maike Bittmann,Harald Gröger
出处
期刊:Chem catalysis
[Elsevier]
日期:2023-04-01
卷期号:3 (4): 100572-100572
被引量:1
标识
DOI:10.1016/j.checat.2023.100572
摘要
An efficient and broadly applicable synthetic method for the industrial product class of aromatic nitriles is presented, which—in contrast to technical methods such as ammoxidation and substitutions with cyanide—proceeds at energy-saving low temperature and avoids the use of highly toxic cyanide. As catalysts, a set of regioselective enzymes based on the aldoxime dehydratase from Rhodococcus sp. N-771 were identified and, in part, designed by a combined experimental and modeling approach. Due to a tailor-made enlarged active site, the variants M29G and F306A converted meta- and para-substituted substrates with even bulky substituents such as phenyl. In addition, the process runs at high substrate loading (of up to 500 mM as demonstrated for a model process) and shows a promising substrate scope.
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