Green fabrication, characterization and antimicrobial activities of AgO/Ag/carboxymethyl chitosan- graphene oxide films

Antimicrobial AgO/Ag/carboxymethyl chitosan-graphene oxide (AgO/Ag/CGO) films were synthesized by UV-light-assisted reduction followed by ionic crosslinking method. The structure of the AgO/Ag/CGO films were explored by X-ray diffraction (XRD), field-emission scanning electron microscope (FE-SEM), Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), ultraviolet–visible spectroscopy (UV–vis), and Fourier-transform infrared (FTIR) analysis. We have also tested the cytotoxicity and cell viability of AgO/Ag/CGO3. The results of cytotoxicity of AgO/Ag/CGO3 on HepG2 cells suggests that the nanocomposite is not cytotoxic. Additionally, thermal stability, swelling ratio, antimicrobial activity of the AgO/Ag/CGO films were evaluated in detail, which revealed that the addition of GO improves the thermal stability and swelling ratio of AgO/Ag/CGO films. Of the three AgO/Ag/CGO films, the AgO/Ag/CGO3 exhibited better antimicrobial activity and higher inhibition rate against E. coli (89.2%) and S. aureus (93.4%). Therefore, the simple and green method is potential candidate for prepare of Ag-based antimicrobial film materials.