化学
DNA甲基化
DNA
表面增强拉曼光谱
表观遗传学
DNA测序
胞嘧啶
拉曼光谱
锆
基因
生物物理学
组合化学
分子生物学
生物化学
基因表达
无机化学
拉曼散射
生物
物理
光学
作者
Yuan Zhang,De-Sheng Zhan,Xiaoying Xu,Zhe Zhang,Mahmoud Elsayed Hafez,Yue He,Yang Li,Da-Wei Li
出处
期刊:Talanta
[Elsevier]
日期:2023-02-01
卷期号:253: 123941-123941
被引量:6
标识
DOI:10.1016/j.talanta.2022.123941
摘要
DNA methylation is an important feature of gene epigenetics that affects the metabolic process of organisms. Although surface-enhanced Raman spectroscopy (SERS) has demonstrated great potential in label-free DNA detection, discriminating the various processes involved in DNA methylation remains a challenge. DNA molecules fold themselves, wrapping the hydrophobic bases, thus making it difficult for traditional methods to detect single-base signals. In this study, we develop a SERS platform for detecting DNA via modifying silver nanoparticles by zirconium ions to obtain the DNA fingerprint information of base methylations (N6-methylated adenine and 5-methylated cytosine). Zirconium ions open the folded DNA molecules, enabling SERS signals of the four DNA bases (A, C, G, T) to be obtained as well as identification of the subtle differences between normal and methylated DNA with single base-level sensitivity. Moreover, the identifying information of DNA methylation was obtained by combining principal component analysis (PCA) with 2D correlation spectroscopy analysis. The findings of this study provide a substantial progress for current platforms for DNA sequencing, genetic testing, and gene-disease treatment.
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