Interplay of electric field and pressure-driven flow inducing microfluidic particle migration

The lateral migration of colloidal particles inside a microfluidic channel has gained attention due to being both fundamentally intriguing and applicable for particle separation, such as cancer cell isolation or extracellular vesicle purification. Applying an external electric field combined with a pressure-driven flow induces such lateral migrations. In this study, new modes of lateral particle migration have been found by experimentally investigating 6 µm particles in the co-presence of electric field and pressure-driven flow. The experiments revealed the importance of the relative strengths of electric field and pressure gradient in determining particle lateral positioning. We hypothesize that the nonuniformity of the polarization caused by the external electric field and the rotation of the particle due to the background pressure-driven flow result in these modes of transverse migration. These new migration patterns are further utilized to perform microparticle separation and, more importantly, present a novel separation modality.