Ecotoxicity study of reduced-Cr(III) generated by Cr(VI) biosorption

Hexavalent chromium (Cr(VI)) is a known carcinogen in living organisms, and many studies have investigated Cr(VI) removal methods. Biosorption, one of the Cr(VI) removal methods, is dominated by chemical binding, ion exchange, physisorption, chelation and oxidation-reduction. Among these mechanisms, Cr(VI) can be removed by nonliving biomass through a redox reaction, which has been recognized as ‘adsorption-coupled reduction’. Cr(VI) is reduced to Cr(III) during biosorption, but there are no studies on the property and toxicity of the reduced-Cr(III). In this study, the harmfulness of reduced-Cr(III) was identified through assessment of mobility and toxicity in nature. Pine bark, a low-cost biomass, was used to remove Cr(VI) from aqueous solution. Reduced-Cr(III) was characterized by structural features using X-ray Absorption Near Edge Structure (XANES) spectra, mobility assessed using precipitation, adsorption and soil column test, and toxicity using radish sprouts and water flea. XANES analysis confirmed that reduced-Cr(III) has an unsymmetrical structure, and the reduced-Cr(III) showed low mobility and was virtually non-toxic, and helpful for plant growth. Our findings demonstrate that Cr(VI) biosorption technology using pine bark is a groundbreaking technology for Cr(VI) detoxification.