去细胞化
材料科学
自愈水凝胶
聚合物
化学工程
组织工程
表面改性
膜
PEG比率
肿胀 的
粘附
生物医学工程
复合材料
高分子化学
化学
工程类
经济
医学
生物化学
财务
作者
Golara Kafili,Elnaz Tamjid,Hassan Niknejad,Abdolreza Simchi
标识
DOI:10.1016/j.eurpolymj.2022.111566
摘要
Recently, decellularized amniotic membrane-derived hydrogels (DAMHs) have received significant attention for wound care, ocular surface reconstruction, and chondral healing. Despite the advantages of DAMHs for tissue engineering (TE), the loss of structural components during the decellularization process mitigates their mechanical strength and thus limits their practical application. Herein, we present a method for the surface modification of two-dimensional nanosilicates (laponite) as a rheological modifier to tailor the properties of DAMHs. Results show that after introducing nanosilicates, severe aggregation of the nanoparticles occurs, owing to the shielding effect of ions on the surface and edges of laponite. Loose interactions between the hydrophobic nanosilicate tactoids and hydrophilic polymer form laminated clay clusters surrounded by collagen fibers. At a high concentration of laponite (1:1 w/w), liquid-solid phase separation may also occur. A decreased storage modulus (up to 80 %), swelling ratio (up to 50 %), and gelation rate (up to 16 %) are thus attained. Electrosteric stabilization of the nanosilicates with amine-terminated polyethyleneglycol (AT-PEG) prevents aggregation of the nanosilicate in the hydrogel matrix and provides uniform distribution. As a result, most of the impaired physicomechanical properties are resolved. In vitro cell studies also determine that the AT-PEG modified nanosilicate-DAMHs exhibit higher cell viability and cell adhesion. The results of this research can pave the way toward developing injectable DAMHs with improved properties for TE.
科研通智能强力驱动
Strongly Powered by AbleSci AI