Objective:To compare some pathogenic substances of Staphylococcus aureus(S.aureus) with enterotoxin B and that of its L form.Methods:Coagulase,heat stable nuclease and enterotoxin B(SEB) generated from S.aureus L form were detected and compared with that from S.aureus .Results:In comparing with original S.aureus ,the L form showed delaying plasma coagulation,smaller in clear ring of heat stable nuclease,but no significantly decrease in optic density(OD) of SEB.Conclusions: S.aureus L form still generated some pathogenic substances,but getting less.