A Comparison Investigation of Coix Seed Oil Liposomes Prepared by Five Different Methods

The physicochemical properties of coix seed oil (CSO) liposomes prepared by five different methods were evaluated for morphology, encapsulating efficiency, particle size, storage stability, and in vitro release. The different preparation methods resulted in several types of vesicles with different properties. The type of vesicles was closely related to leakage pattern, which affected the storage stability and in vitro release profiles. Ethanol injection method was the best choice for preparing safe and stable liposomes with controlled release. The release mechanisms might account for the diffusion of CSO, and Higuchi was the most suitable model for liposomes stored at high temperature or released in simulated intestinal fluid (SIF).