组织工程
脐静脉
自愈水凝胶
生物医学工程
脚手架
细胞
电池类型
肝组织
细胞生物学
血管生成
材料科学
化学
去细胞化
生物
体外
生物化学
医学
癌症研究
高分子化学
内分泌学
作者
Jin Woo Lee,Yeong‐Jin Choi,Woon-Jae Yong,Falguni Pati,Jin‐Hyung Shim,Keonwook Kang,In-Hye Kang,Jaesung Park,Dong‐Woo Cho
出处
期刊:Biofabrication
[IOP Publishing]
日期:2016-01-12
卷期号:8 (1): 015007-015007
被引量:193
标识
DOI:10.1088/1758-5090/8/1/015007
摘要
Several studies have focused on the regeneration of liver tissue in a two-dimensional (2D) planar environment, whereas actual liver tissue is three-dimensional (3D). Cell printing technology has been successfully utilized for building 3D structures; however, the poor mechanical properties of cell-laden hydrogels are a major concern. Here, we demonstrate the printing of a 3D cell-laden construct and its application to liver tissue engineering using 3D cell printing technology through a multi-head tissue/organ building system. Polycaprolactone (PCL) was used as a framework material because of its excellent mechanical properties. Collagen bioink containing three different types of cells—hepatocytes (HCs), human umbilical vein endothelial cells , and human lung fibroblasts—was infused into the canals of a PCL framework to induce the formation of capillary-like networks and liver cell growth. A co-cultured 3D microenvironment of the three types of cells was successfully established and maintained. The vascular formation and functional abilities of HCs (i.e., albumin secretion and urea synthesis) demonstrated that the heterotypic interaction among HCs and nonparenchymal cells increased the survivability and functionality of HCs within the collagen gel. Therefore, our results demonstrate the prospect of using cell printing technology for the creation of heterotypic cellular interaction within a structure for liver tissue engineering.
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