Overexpression of the caryophyllene synthase gene GhTPS1 in cotton negatively affects multiple pests while attracting parasitoids

Abstract BACKGROUD Volatile terpenes can act as ecological signals to affect insect behavior. It has been proposed that the manipulation of terpenes in plants can help to control herbivore pests. In order to investigate the potential pest management function of ( E )‐β‐caryophyllene in cotton plants, the ( E )‐β‐caryophyllene synthase gene ( GhTPS1 ) was inserted into Gossypium hirsutum variety R15 to generate overexpression lines. RESULTS Four GhTPS1‐ transgenic lines were generated, and GhTPS1 expression in transgenic L18 and L46 lines was 3‐5‐fold higher than in R15 plants. The transgenic L18 and L46 lines also emitted significantly more ( E )‐β‐caryophyllene than R15. In laboratory bioassays, L18 and L46 plants reduced pests Apolygus lucorum , Aphis gossypii and Helicoverpa armigera , and attracted parasitoids Peristenus spretus and Aphidius gifuensis , but not Microplitis mediator . In open‐field trials, L18 and L46 plants reduced A. lucorum , Adelphocoris suturalis and H. armigera , but had no significant effects on predators. CONCLUSION Our findings suggest that L18 and L46 plants reduce several major hemipteran and lepidopteran cotton pests, whereas, two parasitoids P. spretus and A. gifuensis , were attracted by L18 and L46 plants. This study shows that overexpressing GhTPS1 in cotton may help to improve pest management in cotton fields. © 2019 Society of Chemical Industry