黄曲霉
迷迭香
黄曲霉毒素
精油
杀菌剂
菌丝体
桉树醇
樟脑
化学
官房
食品科学
最小抑制浓度
大蒜油
寄生曲霉
传统医学
植物
生物
抗菌剂
有机化学
医学
作者
Natália da Silva Bomfim,Cássia Yumie Kohiyama,Lydiana Pollis Nakasugi,Samuel Botião Nerilo,Simone Aparecida Galerani Mossini,Jéssica Cristina Zoratto Romoli,Jane Martha Graton Mikcha,Benício Alves de Abreu Filho,Miguel Machinski
标识
DOI:10.1080/19440049.2019.1678771
摘要
The increased risk to health by diverse pathologies, such as cancer, liver diseases, and endocrine alterations, caused by chemical residues in food, has led to the search for sustainable agricultural management alternatives, such as the use of essential oils for the development of natural and eco-friendly fungicides. The aim of this study was to evaluate the antifungal and antiaflatoxigenic activity of Rosmarinus officinalis L. essential oil (REO) against Aspergillus flavus Link. REO was obtained by hydrodistillation and its major components were identified as 1,8-cineole (eucalyptol, 52.2%), camphor (15.2%) and α-pinene (12.4%) by GC/MS and NMR. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were both 500 µg/mL. REO reduced the mycelial growth of A. flavus at a concentration of 250 µg/mL (15.3%). The results obtained from scanning electron microscopy (SEM) demonstrated a reduction in the size of conidiophores and in the thickness of hyphae in A. flavus caused by treatment with REO (250 µg/mL). The production of ergosterol and the biomass of mycelium were both reduced as the REO treatment concentration increased. The production of aflatoxins B1 and B2 was inhibited after treatment with 250 µg/mL REO, a concentration below the MIC/MFC, indicating that the antiaflatoxigenic effect of REO is independent of its antifungal effect and is likely due to its direct action upon toxin biosynthesis. The data demonstrated that REO may be used as an alternative to synthetic fungicides.
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