Cytidine-gold nanoclusters as peroxidase mimetic for colorimetric detection of glutathione (GSH), glutathione disulfide (GSSG) and glutathione reductase (GR)

Abnormal levels of glutathione (GSH) and glutathione oxidized (GSSG) usually relates to some diseases, thus quantifying the amount of GSH or GSSG is of great significance. A label-free sensing assay based on the enzyme-mimicking property of Cytidine-Au nanoclusters (Cy-AuNCs) was demonstrated for colorimetric detection of GSH, GSSG and glutathione reductase (GR). Firstly, obvious blue color accompanied with an absorption peak at 652 nm was observed due to the high peroxidase-like activity of Cy-AuNCs toward 3,3′,5,5′-tetramethylbenzidine (TMB). Then, in the presence of target, the mimetic activity of Cy-AuNCs could be strongly inhibited and used to achieve the visualization detection. The inhibition effect arose from the surface interaction between GSH and Cy-AuNCs. Linear relationships between absorbance response and concentration were obtained between 0 and 0.4 mM for GSH, 0–2.5 mM for GSSG and 0–0.2 U/mL for GR. The limit of detection (LOD) was calculated as low as 0.01 mM, 0.03 mM and 0.003 U/mL for GSH, GSSG and GR, respectively. Furthermore, the proposed method displayed rapid response, easy procedure and high selectivity.