化学
生物传感器
荧光
信号(编程语言)
酶
纳米技术
生物物理学
生物化学
物理
材料科学
量子力学
计算机科学
生物
程序设计语言
作者
Jin Xu,Minfang Gui,Hongbo Li,Lei Nie,Weihua Zhao,Suqin Wang,Ru‐Qin Yu
标识
DOI:10.1016/j.aca.2024.342581
摘要
Cancer detection is still a major challenge in public health. Identification of oncogene is the first step toward solving this problem. Studies have revealed that various cancers are associated with miRNA expression. Therefore, the sensitive detection of miRNA is substantially important to solve the cancer problem. In this study, let-7a, a representative substance of miRNA, was selected as the detection target. With the assistance of magnetic beads commonly used in biosensors and self-synthesized graphene oxide materials, specificity and sensitivity detection of the target gene let-7a were achieved via protease-free signal amplification. The limit of detection (LOD) was as low as 15.015pM. The fluorescence signal intensity showed a good linear relationship with the logarithm of let-7a concentration. The biosensor could also detect let-7a in complex human serum samples. Overall, this fluorescent biosensor is not only simple to operate, but also strongly specificity to detect let-7a. Therefore, it has substantial potential for application in the early diagnosis of clinical medicine and biological research.
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