生物
秀丽隐杆线虫
破译
热休克蛋白
热冲击
热应力
计算生物学
战斗或逃跑反应
休克(循环)
遗传学
基因
医学
内科学
动物科学
作者
Teresa Rubio‐Tomás,Eva Alegre-Cortés,Eirini Lionaki,José M. Fuentes,Nektarios Tavernarakis
标识
DOI:10.1016/bs.mcb.2024.02.001
摘要
The soil nematode worm Caenorhabditis elegans is a simple and well-established model for the study of many biological processes. Heat shock and thermotolerance assays have been developed for this nematode, and have been used to decipher the molecular relationships between thermal stress and aging, among others. Nevertheless, a systematic and methodological comparison of the different approaches and tools utilized is lacking in the literature. Here, we aim to provide a comprehensive summary of the most commonly used strategies for carrying out heat shock and thermotolerance assays that have been reported, highlighting specific readouts and scientific questions that can be addressed. Furthermore, we offer examples of thermotolerance assays performed with wild type nematodes, that can serve as a gauge of the animal survival under diverse conditions of stress.
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