粘蛋白
粪便
微生物学
聚糖
低聚糖
细菌
化学
大肠
生物
生物化学
糖蛋白
遗传学
作者
Naomichi Nishimura,Hiroki Tanabe,Tatsuya Morita
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 37-44
标识
DOI:10.1007/978-1-0716-3670-1_2
摘要
Since the core protein of mucin in the digesta of the stomach and small intestine, which is less affected by bacteria, remains intact, mucin content can be measured by enzyme-linked immunosorbent assay (ELISA). However, the mucin core protein in bacteria-rich colon digesta and feces is partially hydrolyzed by bacterial enzymes and not fully recognized by mucin antibodies, so mucin cannot be accurately quantified by ELISA. This method quantifies the glycan content linked to the mucin core protein and expresses mucin content in the colon digesta and feces as the equivalent of O-linked oligosaccharide chain. Although mucin glycans are also hydrolyzed by colonic bacteria, this method is a more accurate and simple way to measure mucin content in the digesta of the large intestine and feces than the ELISA method.
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