Wnt信号通路
癌症研究
异位表达
细胞生长
转移
细胞周期
连环素
医学
结直肠癌
细胞凋亡
上皮-间质转换
流式细胞术
癌症
细胞
活力测定
细胞迁移
细胞周期检查点
体内
细胞培养
生物
信号转导
内科学
免疫学
细胞生物学
生物化学
生物技术
遗传学
作者
Kun Yang,Xiaolu Li,Zhongxiang Jiang,Junfeng Li,Qianxi Deng,Jin He,Jun Chen,Xiaoqing Li,Shuman Xu,Zhen Jiang
标识
DOI:10.1016/j.dld.2023.10.026
摘要
Background Colorectal cancer (CRC) is one of the most commonly diagnosed malignant tumours of the digestive tract, and new therapeutic targets and prognostic markers are still urgently required. However, the role and molecular mechanisms of ATP binding cassette subfamily A member 8 (ABCA8) in CRC remain unclear. Methods Databases and clinical specimens were analysed to determine the expression level of ABCA8 in CRC. Colony formation, CCK-8 and Transwell assays were conducted to determine cell proliferation, viability, migration and invasion. Flow cytometry was used to detect cell cycle progression and apoptosis. Western blot and rescue experiments were performed to determine the specific mechanisms of action of ABCA8. Results ABCA8 expression is dramatically down-regulated in CRC tissues and cell lines. Ectopic expression of ABCA8 induced apoptosis and cell cycle arrest in vitro, inhibited cell growth, suppressed migration and invasion, reversed epithelial-mesenchymal transition and suppressed xenograft tumour growth and metastasis in vivo. Mechanistically, ABCA8 inhibited CRC cell proliferation and metastasis through the Wnt/β-catenin signalling pathway, both in vitro and in vivo. Conclusion We verified that ABCA8 inhibits the malignant progression of CRC through the Wnt/β-catenin pathway. This newly discovered ABCA8-Wnt-β-catenin signalling axis is probably helpful in guiding the clinical diagnosis and treatment of CRC.
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