Multi-class analysis of 100 drug residues in cosmetics using high-performance liquid chromatography-quadrupole time-of-flight high-resolution mass spectrometry

Cosmetics are an important aspect of the lives of many people. With an increasing demand for cosmetics, consumers pay more attention to their efficacy and composition. To improve their efficacy, prohibited substances, such as hormones, glucocorticoids, antibiotics, antifungals and antihistamines, may be added to cosmetics. We developed a rapid method for the multi-class analysis of drug residues in toner and lotion cosmetic samples using high-performance liquid chromatography coupled with quadrupole time-of-flight high-resolution mass spectrometry (HPLC-Q-TOF-HRMS). The primary variables in the extraction and purification steps were studied to minimize the interference of the sample matrix. The non-information-dependent sequential window acquisition of all theoretical fragment ion spectra (SWATH®) mode was used to improve the data acquisition efficiency. The secondary product ion peak areas were used for quantification to obtain a satisfactory matrix effects. The validation experiments confirmed that the developed method exhibited good linearity (5-200 ng/L) with correlation coefficients (R) ≥ 0.9902. Our developed method was then successfully applied to 92 real cosmetic samples. The calibration curve established by this method can be used for retrospective quantitative analysis over long durations without re-calibration. This method is efficient and suitable for screening and controlling multi-class prohibited substances in the cosmetics industry to reduce potential risks.