Effects of dietary supplementation levels of vitamin A and vitamin D3 on growth performance, jejunal function, and tibia development in goslings from 1 to 28 days of age

This study explored the interaction effects of dietary Vitamin A (VA) and Vitamin D3 (VD3) on growth performance, jejunal function, and tibia development in goslings, aiming to identify any synergistic outcomes that may reshape nutritional strategies for geese production. A total of 540 one-day-old male Jiangnan White goslings with similar body weight (82 ± 5 g) were randomly assigned into 9 treatments with five replicate pens per treatment and 12 birds per pen. The bird trial employed a 3 × 3, two-factorial treatment with three levels of VA (5000, 7000, and 9000 IU/kg) and three levels of VD3 (1000, 1500, and 2000 IU/kg) from one to 28 days of age. Main effects analysis indicated that birds fed 7000 IU/kg VA exhibited the highest ADG, BW, jejunal maltase activity and IL-10 content (P < 0.05), while 9000 IU/kg VA had the highest SOD activity and content of IL-6 and TNF-α in jejunal mucosa (P < 0.05). Both 7000 IU/kg or 9000 IU/kg VA increased the jejunal IL-1β content, relative expression of tight junction protein 1 (TJP1) mRNA, tibia defatted weight and ash weight (P < 0.05). Birds fed 2000 IU/kg VD3 exhibited the highest ADFI, while both 1500 or 2000 IU/kg VD3 increased jejunal maltase activity, and tibia ash content (P < 0.05). An interaction between VA and VD3 on ADFI, F/G, jejunal maltase activity, mucosal immune factors (IL-1β, IL-6, IL-10, TNF-α), tibia ash content, and bone morphogenetic protein-2 (BMP-2) expression. A simple effects analysis revealed that at a 5000 IU/kg VA, adding 1000 IU/kg VD3 decreased IL-1β, IL-6, TNF-α (P < 0.05). At a 7000 IU/kg VA, adding 1500 or 2000 IU/kg VD3 decreased TNF-α, and increased jejunal maltase activity(P < 0.05). At a 9000 IU/kg VA, adding 1000 IU/kg VD3 decreased ADFI, F/G, jejunal maltase activity, tibia ash, and BMP-2, while IL-1β, IL-6, and TNF-α increased (P < 0.05). At a 9000 IU/kg VA, adding 2000 IU/kg VD3 increased IL-10 (P < 0.05). At a 1000 IU/kg VD3, adding 5000 IU/kg VA increased F/G, jejunal maltase activity and IL-10, while decreased IL-1β, IL-6, TNF-α (P < 0.05), and adding 9000 IU/kg VA decreased tibia ash and BMP-2 (P < 0.05). At 1500 or 2000 IU/kg VD3, adding 7000 IU/kg VA increased jejunal maltase activity, IL-10 (P < 0.05). At a 2000 IU/kg VD3, adding 9000 IU/kg VA increased IL-6, and TNF-α (P < 0.05). In summary, a dietary level of 7000 IU/kg of VA and 2000 IU/kg of VD3 can be a balanced combination to optimize feed intake and conversion, jejunal function, and tibia mineralization, consequently enhancing growth performance in goslings.