Split aptamer remodeling-initiated target-self-service 3D-DNA walker for ultrasensitive detection of 17β-estradiol

脱氧核酶 适体 DNA 检出限 生物传感器 化学 A-DNA 信号(编程语言) 纳米技术 生物系统 计算机科学 色谱法 材料科学 分子生物学 生物化学 生物 程序设计语言
作者
Shuo Qi,Xiaoze Dong,Yuhan Sun,Yin Zhang⋆,Nuo Duan,Zhouping Wang
出处
期刊:Journal of Hazardous Materials [Elsevier BV]
卷期号:439: 129590-129590 被引量:41
标识
DOI:10.1016/j.jhazmat.2022.129590
摘要

DNA walker machines, as one of the dynamic DNA nanodevices, have attracted extensive interest in the field of analysis due to their inherent superiority. Herein, we reported a split aptamer remodeling-initiated target-self-service 3D-DNA walker for ultrasensitive, specific, and high-signal-background ratio determination of 17β-estradiol (E2) in food samples. Two split probes (STWS-a and STWS-b) were rationally designed that can undergo structural reassembled to serve as walking strands (STWS) under the induction of the target. Meanwhile, an intact E6-DNAzyme region was formed and activated at the tail of STWS. The activated E6-DNAzyme then continuously drives the 3D-DNA walker for signal amplification and specific detection of E2. Under optimal conditions, the proposed DNA walker-based biosensor exhibited excellent linearity in the range of 1 pM to 50 nM with a low limit of detection (LOD) of 0.28 pM, and good precision (2.7%) for 11 replicate determinations of 1 nM of E2. Furthermore, the developed DNA walker-based biosensor achieved excellent sensitive analysis of E2 in the complex food matrix with recoveries of 95.6-106.5%. This newly proposed split aptamer-based strategy has the advantages of ultrasensitive, high signal-to-background ratio, and high stability. Noteworthy, the successful operation of the DNA walker initiated by the split aptamer expands the principles of DNA walker design and provides a universal signal amplification platform for trace analysis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
槐序零玖完成签到,获得积分10
1秒前
duonicola完成签到,获得积分10
1秒前
2秒前
今后应助wujingshuai采纳,获得30
2秒前
科目三应助haha采纳,获得10
3秒前
3秒前
3秒前
3秒前
王小静完成签到,获得积分10
4秒前
Akim应助YYGQ采纳,获得10
4秒前
5秒前
cchy发布了新的文献求助10
5秒前
5秒前
6秒前
6秒前
zl完成签到,获得积分10
7秒前
xlao发布了新的文献求助10
9秒前
9秒前
9秒前
马仔酷酷地完成签到,获得积分10
10秒前
jisoo完成签到,获得积分20
10秒前
Doris_29发布了新的文献求助10
11秒前
狗蛋完成签到,获得积分10
12秒前
aqiuyuehe发布了新的文献求助10
14秒前
14秒前
14秒前
唐诗阅完成签到,获得积分10
15秒前
刘雪松完成签到,获得积分10
15秒前
xlao完成签到,获得积分10
16秒前
每天都是新的一天完成签到,获得积分10
17秒前
HY发布了新的文献求助10
19秒前
19秒前
科研废物发布了新的文献求助10
19秒前
20秒前
wem发布了新的文献求助30
21秒前
本凡完成签到,获得积分10
21秒前
21秒前
陆晓亦完成签到,获得积分10
22秒前
22秒前
机灵的初晴关注了科研通微信公众号
23秒前
高分求助中
Africanfuturism: African Imaginings of Other Times, Spaces, and Worlds 3000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 2000
Electron microscopy study of magnesium hydride (MgH2) for Hydrogen Storage 1000
Structural Equation Modeling of Multiple Rater Data 700
 Introduction to Comparative Public Administration Administrative Systems and Reforms in Europe, Third Edition 3rd edition 590
全球膝关节骨性关节炎市场研究报告 555
Exhibiting Chinese Art in Asia: Histories, Politics and Practices 540
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3893280
求助须知:如何正确求助?哪些是违规求助? 3436283
关于积分的说明 10798633
捐赠科研通 3161673
什么是DOI,文献DOI怎么找? 1746211
邀请新用户注册赠送积分活动 843248
科研通“疑难数据库(出版商)”最低求助积分说明 787160