细胞周期蛋白依赖激酶1
生物
细胞周期蛋白依赖激酶
甲型流感病毒
病毒复制
激酶
病毒学
细胞周期
病毒
细胞生物学
化学
细胞
生物化学
作者
Lei Zhao,Yunzheng Yan,Qingsong Dai,Zihao Wang,Ji‐Ye Yin,Yijie Xu,Zhuang Wang,Xiaojia Guo,Wei Li,Ruiyuan Cao,Wu Zhong
标识
DOI:10.1016/j.antiviral.2022.105296
摘要
Many viruses use the host cell division cycle to facilitate replication. Cyclin-dependent kinases (CDKs) are a group of serine/threonine kinases that play a central role in regulating cell cycle progression. However, the prospect of using CDKs for anti-influenza virus treatment remains to be elucidated. We conducted this study to investigate the potential of the CDK1 inhibitor Ro-3306 in preventing influenza virus infection and to elucidate the underlying mechanism. We showed that Ro-3306, a CDK1 inhibitor, exerts anti-influenza activity both in vitro and in vivo. Proof-of-concept studies revealed that knockdown of host CDK1 might affect the splicing of M2 viral mRNA, leading to the restriction of viral replication. Moreover, Ro-3306 directly bound to viral PB2 protein and inhibited viral RNA replication. Transcriptome analysis further revealed that Ro-3306 treatment inhibited the expression of MAPK-regulated genes, which might also contribute to the antiviral activity of Ro-3306. This study highlighted the multifunctional role of Ro-3306 as a novel anti-influenza virus agent.
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