核型
白血病
淋巴细胞白血病
细胞培养
急性淋巴细胞白血病
生物
癌症研究
分子生物学
T细胞
免疫学
细胞
基因
遗传学
染色体
免疫系统
作者
Ruth A. Gjerset,Alice L. Yu,Martin Haas
出处
期刊:PubMed
日期:1990-01-01
卷期号:50 (1): 10-4
被引量:5
摘要
We have devised methods facilitating the establishment of continuous cultures of T-cell blasts from patients with acute lymphoblastic leukemia of T-cell type at diagnosis. The cultured cells closely resemble those of the patients at the time of diagnosis with respect to surface markers, karyotype, and T-cell receptor gene rearrangements. Cultured T-cell acute lymphoblastic leukemia (diagnosis) cells (a) are lymphocytes with a convoluted nucleus; (b) have doubling times of 24-48 h; (c) are dependent for growth on interleukin 2; (d) are reverse transcriptase negative; (e) do not form colonies in methyl cellulose; and (f) are clonal with respect to T-cell receptor beta chain rearrangements. Three T-cell acute lymphoblastic leukemia cultures had a normal diploid karyotype, and one had a 6q- deletion which was also present at the time of diagnosis.
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