自愈水凝胶
抗菌活性
细胞粘附
高分子化学
伤口愈合
材料科学
PEG比率
聚合
脚手架
聚乙二醇
粘附
生物医学工程
化学
乙二醇
胶粘剂
有机化学
聚合物
纳米技术
细菌
复合材料
医学
财务
图层(电子)
生物
免疫学
经济
遗传学
作者
Airong Song,Aboli A. Rane,Karen L. Christman
标识
DOI:10.1016/j.actbio.2011.10.004
摘要
The ideal wound-healing scaffold should provide the appropriate physical and mechanical properties to prevent secondary infection, as well as an excellent physiological environment to facilitate cell adhesion, proliferation and/or differentiation. Therefore, we developed a synthetic cell-adhesive polypeptide hydrogel with inherent antibacterial activity. A series of polypeptides, poly(Lys)x(Ala)y (x + y = 100), with varied hydrophobicity via metal-free ring-opening polymerization of NCA-Lys(Boc) and NCA-Ala monomers (NCA = N-carboxylic anhydride) mediated by hexamethyldisilazane (HMDS) were synthesized. These polypeptides were cross-linked with 6-arm polyethylene glycol (PEG)-amide succinimidyl glutarate (ASG) (Mw = 10 K) to form hydrogels with a gelation time of five minutes and a storage modulus (G′) of 1400–3000 Pa as characterized by rheometry. The hydrogel formed by cross-linking of poly(Lys)60(Ala)40 (5 wt.%) and 6-arm PEG-ASG (16 wt.%) (Gel-III) exhibited cell adhesion and cell proliferation activities superior to other polypeptide hydrogels. In addition, Gel-III displays significant antibacterial activity against Escherichia coli JM109 and Staphylococcus aureus ATCC25923. Thus, we have developed a novel, cell-adhesive hydrogel with inherent antibacterial activity as a potential scaffold for cutaneous wound healing.
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