Synthesis of Substituted Benzylamino‐ and Heterocyclylmethylamino Carbodithioate Derivatives of 4‐(3H)‐Quinazolinone and their Cytotoxic Activity

Abstract A new series of substituted benzylamino‐ and heterocyclylmethylamino carbodithioate derivatives of 4‐(3 H )‐quinazolinone were synthesized via four steps starting from 2‐amino‐5‐methylbenzoic acid and initially screened against A‐549 (human non‐small cell lung cancer), HCT‐8 (human colon cancer), and Bel‐7402 (human liver cancer) cell lines at the single concentration of 5 μg/mL using the colorimetric MTT assay. The IC 50 values were determined for the compounds reaching ⪈70% inhibition in primary screening by serial dilution. Among the newly synthesized compounds, 9n exhibited potent in vitro cytotoxicity against A‐549, HCT‐8, and Bel‐7402 cell lines with the IC 50 values of 1.65, 0.93, and 1.43 μM, respectively.