老茧
萝卜
动素
植物
下胚轴
开枪
小苗
Murashige和Skoog培养基
生物
醋酸
蔗糖
园艺
化学
组织培养
食品科学
生物化学
体外
作者
Shigeo Matsubara,Hegazi H. Hegazi
出处
期刊:Hortscience
[American Society for Horticultural Science]
日期:1990-10-01
卷期号:25 (10): 1286-1288
被引量:12
标识
DOI:10.21273/hortsci.25.10.1286
摘要
Callus initiation and growth and plantlet regeneration were studied using eight cultivars of Raphanus sativus L., including six Japanese radishes, one Chinese and one small `Comet' radish. The basal medium was composed of Murashige and Skoog inorganic salts, 2.0 mg myo-inositol/liter, 0.5 mg each of nicotinic acid and pyridoxine·HCl/liter, and 0.1 mg thiamine·HCl/liter, 30 g sucrose and 2 g Gelrite/liter. High callus yields were obtained on basal medium containing (mg·liter -1 ) 0.1 2,4-D and 1.0 BA for two Japanese radishes and 0.1 NAA and 1.0 kinetin for `Comet' radish. Shoots were regenerated from callus by subculturing on basal medium containing 0.1 or 1.0 mg BA/liter and then transferring to basal medium. Rooting occurred on basal medium. Although callus was obtained in all eight cultivars, shoots and plantlets were regenerated only from `Moriguchi', `Nerima Shirinaga', and `Comet'. Chemical names used: 2-(l-naphthyl) acetic acid (NAA); N -(phenylmethyl)-l H -purine-6-amine (BA); 2,4-dichlorophenoxy acetic acid (2,4-D); 6-(furfurylamino)purine (kinetin).
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