异种移植
抗体
免疫学
生物
外周血单个核细胞
抗原
流式细胞术
病毒学
分子生物学
移植
医学
体外
生物化学
外科
作者
Qi Li,Sahar Shaikh,Hayato Iwase,Cassandra Long,Whayoung Lee,Zhongqiang Zhang,Yi Wang,David Ayares,D. K. C. Cooper,Hidetaka Hara
摘要
Abstract Background Old World non‐human primates (OWNHPs) are used for preclinical pig‐to‐NHP studies. However, like pigs, OWNHPs express Neu5Gc, and therefore do not develop natural anti‐Neu5Gc antibodies. New World NHPs (NWNHPs) have been reported not to express Neu5Gc. We investigated the potential of NWNHPs in xenotransplantation research. Methods We investigated expression of Gal, Neu5Gc, and Sd a antigens on RBCs and PBMCs from humans, selected OWNHPs, and capuchin monkeys (a NWNHP). Serum anti‐Gal and anti‐Neu5Gc IgM and IgG levels were measured by ELISA. Binding of primate serum IgM and IgG to pig RBCs was measured by flow cytometry. Results (a) Neither humans, OWNHPs, or capuchin monkeys expressed Gal on their RBCs, but capuchins expressed Gal on PBMCs. Humans and capuchins did not express Neu5Gc on either RBCs or PBMCs, but OWNHPs expressed Neu5Gc on both cells. Sd a was not expressed on any RBCs or PBMCs. (b) By ELISA, human and OWNHP, but not capuchin, sera showed IgM and IgG binding to Gal. Human and capuchin, but not OWNHP, sera demonstrated some binding to Neu5Gc. (c) Anti‐Sd a IgM/IgG antibodies were detected in OWNHP sera. Knockout of Sd a on pig RBCs did not significantly reduce human and capuchin antibody binding. Conclusion Capuchin monkeys could be surrogates for humans in experiments using RBCs, islets, neuronal cells, etc, from triple‐knockout pigs (but may be too small to be used as recipients of pig organ grafts).
科研通智能强力驱动
Strongly Powered by AbleSci AI