Conditional Knockout Adam10 Gene of Neuronal Cell in Mouse Leads to Cortical Dysplasia

Objective: To investigate the role of Adam10 gene in mouse central nervous system(CNS) development. Methods: Gfapcremice were mated to Adam10lox/loxmice using cre-loxp conditional gene knockout technique,and mice of conditional knockout Adam10 gene of neuronal cells(Gfapcre-Adam10lox/lox) were produced. At postnatal day of fourteen, mouse brain sections were prepared and HE staining was used to observe CNS development in Adam10 gene knockout mice. Results: By conditional knockout Adam10 gene of neuronal cell in mice(Gfapcre-Adam10lox/lox), mice could survive to about three weeks after birth. A tiny fraction of mice showed cortical defect,and developed cerebral cortex showed disorders of cortical lamination by HE staining. Conclusion: Adam10 gene plays an important role in CNS development. Conditional knockout Adam10 gene of neuronal cell in mouse leads to cortical defect or dysplasia.