Nucleation in supersaturated solution of tetradecyl hydrogen maleate has been investigated in this paper. The nucleation rate as indicated by induction period are measured. The effects of temperature, supersaturation on the nucleation rate in non-agitated system are reported. The interfacial tension, energy of formation and critical radius of muclei crystal are calculated based on classical nucleation theory. The nucleation rate of THM crystal increase with the rise of the temperature and supersaturation.We found lower temperature range is favourable to the growth of THM singal crystal.